APC anti-human CD3 Antibody (Clone: HIT3a)

评测依据

• review_count：3200
• rating_avg：4.8
• brand_reputation：0
• merchant_count：0
• price_range：[800,1999]
• dimension：best_value
• sources：["京东","天猫","BioLegend官网","小红书"]
• time_range：{"from":"2026-06-25 15:25:19","to":"2026-06-25 15:25:19"}
• intros：{"brand_intro":"BioLegend成立于2002年，总部位于美国加州圣地亚哥，是全球领先的流式细胞术抗体及试剂供应商。公司以\"卓越品质、创新驱动\"为核心理念，拥有超过10000种抗体和试剂产品，覆盖免疫学、肿瘤学、神经科学等领域。其核心技术在于抗体克隆的严格筛选与验证，确保每批产品的高特异性和低批次间差异。创始人兼CEO Gene Lay是一位华裔科学家，致力于推动免疫学研究工具的可及性。BioLegend的品控体系通过ISO 13485认证，所有抗体均经过流式细胞术、ELISA等多平台验证，深受全球科研机构信赖。","product_intro":"APC anti-human CD3 Antibody (Clone: HIT3a) 是BioLegend的经典流式抗体产品，专为人类T细胞表面CD3分子检测设计。该抗体采用高纯度APC荧光染料标记，具有极低的非特异性结合和优异的光稳定性，适用于多色流式分析。核心卖点包括：克隆HIT3a经国际验证，与CD3ε链高亲和力结合；每批次均通过流式细胞术功能测试，确保染色指数稳定。适用场景涵盖免疫表型分析、T细胞活化研究、肿瘤免疫微环境评估等。相比竞品（如BD Pharmingen同类产品），BioLegend在价格上更具竞争力，且提供更灵活的包装规格（25μg/100μg/500μg），适合不同规模实验需求。","key_specs":{"荧光染料":"APC (Allophycocyanin)，激发波长633nm，发射波长660nm，适用于红色激光器","克隆号":"HIT3a，小鼠IgG2a亚型，特异性识别人类CD3ε链","规格":"100μg/支，浓度0.5mg/mL，溶于PBS缓冲液（含0.09% NaN3）","适用样本":"人外周血单个核细胞（PBMC）、全血、组织单细胞悬液","存储条件":"2-8°C避光保存，避免冻融，有效期12个月","交叉反应":"与恒河猴CD3有交叉反应，适用于非人灵长类研究"}}
• alt_products：[{"product_name":"APC anti-human CD3 Antibody (Clone: HIT3a)","brand":"BioLegend","model":"300312","price":1680},{"product_name":"VioBlue anti-human CD45 Antibody (Clone: 5B1)","brand":"Miltenyi Biotec","model":"130-113-114","price":1750},{"product_name":"Alexa Fluor 488 anti-human CD8a Antibody (Clone: RPA-T8)","brand":"Thermo Fisher Scientific","model":"53-0087-42","price":1850}]
• kol_quotes：["@免疫学博士小张（小红书）：\"BioLegend的HIT3a是我做T细胞实验的定海神针，荧光强度吊打很多竞品。\"","@流式老司机（丁香通）：\"APC通道选HIT3a准没错，批次间差异小到可以忽略。\""]
• scoring_breakdown：[{"source":"word_of_mouth","score":94.1,"weight":0.15,"confidence":0.8,"details":{"source":"word_of_mouth_scores","summary":"综合京东、天猫、丁香通及小红书用户反馈，该抗体获得广泛好评。优点：1）染色亮度高，信噪比优异，在多色panel中与其他荧光染料兼容性好；2）批次间一致性高，重复实验数据可靠；3）价格适中，性价比优于BD和Thermo Fisher同类产品。缺点：1）部分用户反映APC荧光在固定后略有衰减，建议使用前优化固定方案；2）对于低表达样本（如某些肿瘤细胞系），可能需要更高浓度或改用PE标记版本。专业评测指出，HIT3a克隆在T细胞亚群分析中表现稳定，尤其适合CD4/CD8双染实验。小红书科研博主推荐其为\"流式入门必备抗体\"，京东好评率98%。","source_count":4}},{"source":"objective","score":74.51665137614678,"weight":0.6,"confidence":0.5,"details":{"rating_avg":4.8,"price":1680,"wom_score":94.1}},{"source":"user_review","score":96,"weight":0.15,"confidence":1,"details":{"rating_avg":4.8,"review_count":3200,"source":"product_reviews_aggregate"}},{"source":"expert","score":82,"weight":0.1,"confidence":0.6,"details":{"kol_count":2,"scored_count":2,"sample":["@免疫学博士小张（小红书）：\"BioLegend的HIT3a是我做T细胞实验的定海神针，荧光强度吊打很多竞品。\"","@流式老司机（丁香通）：\"APC通道选HIT3a准没错，批次间差异小到可以忽略。\""]}}]
• method_version：v2
• search_visual：null