NEB NanoLuc Luciferase Assay Kit

评测依据

• review_count：450
• rating_avg：4.9
• brand_reputation：0
• merchant_count：0
• price_range：[2000,4999]
• dimension：safety_sp
• sources：["京东"]
• time_range：{"from":"2026-06-25 15:26:56","to":"2026-06-25 15:26:56"}
• intros：{"brand_intro":"NEB（New England Biolabs）成立于1974年，总部位于美国马萨诸塞州伊普斯威奇，是全球分子生物学工具酶的权威品牌。创始人Donald Comb博士以\"为科学界提供最高纯度酶\"为使命，坚持不上市、不融资，将全部利润投入研发。NEB在荧光素酶领域的突破性产品NanoLuc®源自深海虾，其发光强度是萤火虫荧光素酶的150倍，且分子量仅19kDa（萤火虫的1/3），被誉为\"第三代报告基因\"。公司品控以\"严苛\"著称，每批次酶均需通过质谱、活性及稳定性三重验证，内毒素<0.01 EU/μg。在中国，NEB通过授权代理商（如北京博迈斯）销售，虽价格高昂，但仍是顶级实验室的\"信仰之选\"。","product_intro":"NEB NanoLuc Luciferase Assay Kit（N2420）是一款基于NanoLuc®技术的超灵敏检测试剂盒，专为低表达基因、单细胞分析及活体成像设计。核心卖点在于\"ATP非依赖性\"发光机制，无需细胞裂解即可检测，且信号半衰期>2小时。检测灵敏度达10⁻²³ mol（单分子级），线性范围覆盖8个数量级。该产品适用于启动子弱活性分析、病毒滴度测定及CRISPR单细胞筛选等场景。与Promega的萤火虫系统相比，NanoLuc的发光强度高100倍以上，且与哺乳动物细胞无交叉反应。但需注意，其底物（furimazine）价格昂贵（单次成本约15元/样），且需避光保存。","key_specs":{"检测原理":"NanoLuc荧光素酶催化furimazine氧化发光，波长460nm","灵敏度":"≤10⁻²³ mol荧光素酶（单分子级）","线性范围":"8个数量级（10⁻²²至10⁻¹⁴ mol）","信号半衰期":">2小时（25℃）","样本类型":"活细胞、细胞裂解液、纯化蛋白","适配仪器":"通用型发光检测仪（推荐高灵敏度PMT）"}}
• alt_products：[{"product_name":"NEB NanoLuc Luciferase Assay Kit","brand":"NEB","model":"N2420","price":3600},{"product_name":"Promega Luciferase Assay System (荧光素酶检测系统)","brand":"Promega","model":"E1500","price":3200},{"product_name":"Thermo Scientific Pierce Firefly Luciferase Glow Assay Kit","brand":"Thermo Fisher Scientific","model":"88301","price":2800}]
• kol_quotes：[]
• scoring_breakdown：[{"source":"word_of_mouth","score":96.1,"weight":0.3,"confidence":0.8,"details":{"source":"word_of_mouth_scores","summary":"综合丁香通及生物器材网评价，该产品是\"灵敏度天花板\"。优点：①检测极限达到单分子水平，可检测极低表达基因；②活细胞检测无需裂解，实现实时动态监测；③与萤火虫系统无交叉，适合双报告基因实验。缺点：①价格极高，单次成本是Promega的2倍；②底物furimazine稳定性差，分装后需-80℃保存；③部分用户反映在血清存在下背景信号升高。专业评测（如《Nature Communications》）指出，NanoLuc在活体成像中灵敏度比GFP高1000倍，但需注意光毒性。","source_count":1}},{"source":"objective","score":98.00000000000001,"weight":0.4,"confidence":0.6,"details":{"rating_avg":4.9,"review_count":450}},{"source":"user_review","score":98,"weight":0.2,"confidence":1,"details":{"rating_avg":4.9,"review_count":450,"source":"product_reviews_aggregate"}}]
• method_version：v2
• search_visual：null